![]() Purple Color, round, grapelike structure. In a 100x magnification the color of bacteria was PURPLE and the shape is ROUND, GRAPE LIKE STRUCTURE. The other cultured bacteria was gram stain using same procedure at Number 4 The second streak culture was subjected to microscopic test. The Unknown bacteria is Enterobacter aerogenes To determine the ability of micro organism to detect presence of catalaseĪ selective test for Enteric bacteria that produces evident colonies To determine the ability of micro organism to detect Urease To determine the ability of the organism to split indole from tryptophan To determine the ability of organism to detect enzyme Citrase To determine if bacteria produce gelatinase on fermentation To determine if bacteria can breakdown casein and absorb amino acid on fermentation The Staphylococcus aureus and Enterococcus faecalis are positive to mannitol. The following bacteria are negative to mannitol Bacillus cereus, Bacillus Subtilis, ,Staphylococcus Epidermidis. To determine bacteria that grows in high salt To determination gram reaction of the bacteriumĬrystal violet, distilled water, Iodine, Alcohol Safranin The save NA was labeled Gram Negative Bacteria was subject to the following test.The bacteria was colored PINKEST RED and the shape is ROD SHAPED which are the characteristic features of a GRAM NEGATIVE BACTERIA. The air dried slide was viewed first at 10X magnification, then to a 100X magnification with the immersion oil to cover the slide. The mixture was air dried and ready for Microscopic examination. A safranin was drop to the mixture to cover the colorless area and then washed again with distilled water. Then the mixture was soaked in Iodine for 1 min. After 30 sec the mixture was rinse with distilled water, taking extra care not to hit the mixture. The purpose of this is to stain the bacteria. The mixture was soaked with Crystal violet for 30 sec. The mixture of water and Unknown specimen A ) was air dried, then passed lightly 3 times in a heat so that the mixture will stick on the slide. The purpose of the water is to make a pool so that the Unknown specimen can be transferred and spread in the slide. Using a sterilized inoculating loop a drop distilled water in a dry glass slide which was previously soaked in alcohol. These 2 NA plate was incubated to 37 C to grow for next meeting.Īfter 2 days the 2 different NA plates were examined and separately transferred into the microscopic slide to be examine which is the Gram (-) and the gram (+) by using the methods Gram Staining (Tortora, et al. ![]() It helps to isolate a possible a gram (+) and a gram (-). The purpose of this is to grow separately the 2 possible bacteria. With a sterilized inoculating loop the colony was separately inoculated to a new set of NA for growth Taking extra care on label and streaking. Since there were 2 distinct color and colony in the NA plate.
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